Gintama Episode 80: A Person That Wears Glasses Suddenly Takes the Glasses Off, It Feels Like Something Is Missing. Like the Part of the Person Is Missing.
Episode 80 of 201 of Gintama, aired November 7, 2007, 25 minutes. Available with English subtitles and dub where released.
Episode summary
The battle between Gintoki and Binbokusai begins; meanwhile, Shinpachi discovered that Kyubei was girl.